Reference number
ISO 12966-3:2016
© ISO 2024
International Standard
ISO 12966-3:2016
Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH)
Edition 2
2016-05
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Published (Edition 2, 2016)
This publication was last reviewed and confirmed in 2021. Therefore this version remains current.

ISO 12966-3:2016

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CHF 42
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Abstract

ISO 12966-3:2016 specifies a rapid base-catalysed transesterification method for fats and oils with trimethylsulfonium hydroxide (TMSH) to prepare fatty acid methyl esters. The method is exclusively applicable to the preparation of methyl esters of fats and oils for gas liquid chromatographic (GLC) analysis. It is applicable to all fats and oils, but excluding those coming from milk and milk products. Isomerization of unsaturated fatty acids only occurs to a minor extent and isomerized fatty acids are only present at the determination limit. As isomerization takes place, the procedure is not recommended for conjugated linoleic acid (CLA).

Only about 70 % to 80 % of the free fatty acids are esterified. In the case of conjugated cyclopropyl and cyclopropenyl fatty acids, side reactions may occur, but these do not interfere with the determination of the fatty acids.

General information

  • Status
     : Published
    Publication date
     : 2016-05
    Stage
    : International Standard confirmed [90.93]
  • Edition
     : 2
    Number of pages
     : 4
  • Technical Committee :
    ISO/TC 34/SC 11
    ICS :
    67.200.10 
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