ISO/TS 20224-3:2020

Published (Edition 1, 2020)

This publication was last reviewed and confirmed in 2025. Therefore this version remains current.

ISO/TS 20224-3:2020

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Abstract

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of porcine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of porcine material derived from pig (Sus scrofa domesticus) and wild boar (Sus scrofa).

The target sequence is a partial fragment of the Sus scrofa beta actin (ACTB) gene, partial cds. (i.e. GenBank accession number DQ452569.1)^[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD_{95 %}).

General information

Status
: Published

Publication date
: 2020-07

Stage
: International Standard confirmed [90.93]
Edition
: 1

Number of pages
: 17
Technical Committee :
ISO/TC 34/SC 16

ICS :
67.050
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